New Ring Detection: SCI Papers Published
Fenobucarb (2-sec-butylphenylmethylcarbamate, BPMC) is a carbamate insecticide. Widely used in grasslands and farmland to protect crops from plague. Its careless use may cause plant contamination, and grazing animals may accumulate BPMC residues in their muscle tissue, milk and eggs. This increases the risk to human health through the food chain.
The potential risks and toxic mechanisms of BPMC for animals and humans have not been fully elucidated. The new loop detection was studied using mature zebrafish technology. The results of the December 27, 2018 study successfully published the SCI paper "Fenobucarb induces heart failure and cerebral hemorrhage in zebrafish".
In this study, 48 hpf (48 hours after fertilization) to 72 hpf zebrafish embryos were exposed to various concentrations of BPMC. We found that BPMC induced severe heart failure with bradycardia, cardiac dilatation, decreased contraction, decreased cardiac output and slowed blood flow, and cardiomyocyte apoptosis.
BPMC causes zebrafish heart dilatation and venous congestion
BPMC-induced apoptosis of zebrafish cardiomyocytes
BPMC also induced cerebral hemorrhage and red blood cell reduction in a dose-dependent manner, and increased ROS production and capase 9 and 3/7 activation were also observed. ATPase-related gene (atp2a1l), calcium channel-related gene (cacna1ab), sodium channel-related gene (scn5Lab), potassium channel-related gene (kcnq1), and cardiac troponin C regulatory gene (tnnc1a) mRNA in zebrafish after BPMC exposure Levels and several apoptosis-related genes were significantly down-regulated.
BPMC treatment significantly induced zebrafish cerebral hemorrhage
We found that the expression of all these marker genes in zebrafish cardiac function or development was significantly reduced after BPMC exposure. These new findings suggest that exposure to BPMC is a possible risk factor for cardiovascular and cerebrovascular systems, and further studies of the effects of BPMC exposure on other animal models are needed to confirm these findings.
Significant down-regulation of cardiac function or development-related gene expression in zebrafish after BPMC exposure
Original: Aquatic Toxicology 209 (2019) 34–41. https://doi.org/10.1016/j.aquatox.2018.12.020 .